Effect of Plant Growth Regulators on the Production of Flavonoids by Cell Suspension Cultures of Digitalis Lanata
نویسندگان
چکیده
In this paper we investigated the effect of some phytohormones on the production of flavonoids in cell suspension cultures of Digitalis lanata. There were used two cell lines and different combinations and concentrations of phytohormones. The highest flavonoids amounts were obtained after 14 days of treatment. Among the tested combinations, the highest yield of flavonoids, 7.58 mg/g d.w. (dry weight) was obtained, for line 13C-100, with 0.1 mg/L IAA (β-indoleacetic acid) + 1 mg/L BA (6-benzylaminopurine). For line 11, the most significant increase in flavonoid amount was achieved with 0.1 mg/L NAA (α-naphtaleneacetic acid) + 1 mg/L BA.
منابع مشابه
Effect of light and passage on cardiac glycoside production on cultured cell of Digitalis nervosa Steud & Hochst
Light is important for the production of metabolites by plant cell culture. It is therefore necessary to evaluate the light conditions with respect to exposure time. Both enhancement and inhibition have been observed depending on exposure time and species. Callus culture of D. nervosa was established and effect of light and passage on cardiac glycoside production was studied. In vitro cultures ...
متن کاملEffect of light and passage on cardiac glycoside production on cultured cell of Digitalis nervosa Steud & Hochst
Light is important for the production of metabolites by plant cell culture. It is therefore necessary to evaluate the light conditions with respect to exposure time. Both enhancement and inhibition have been observed depending on exposure time and species. Callus culture of D. nervosa was established and effect of light and passage on cardiac glycoside production was studied. In vitro cultures ...
متن کاملمطالعه باززایی مستقیم و غیر مستقیم در گونههای Digitalis lanata و D. purpurea در شرایط درون شیشه
Foxglove (Digitalis sp.), as an ornamental and medicinal plant, is used for treatment of heart congestive disorders. Use of plant cell and tissue culture can be effective in large scale micropropagation and stable production of medicinal compounds. In this study direct and indirect regeneration methods in two species of this genus (D. purpurea and D. lanata) were investigated in response to dif...
متن کاملتولید و نگهداری کشت سلولی گیاه Digitalis nervosa Steud & Hochst و بررسی متابولیتهای ثانویه آن
Background and purpose: Digitalis nervosa is a wild species growing in Tran and the major cardenolode of this species is lanatoside Â. D nervosa cell culture was estabilished and its secondary metabolites were studied. Materials and methods : Seeds of D. nervosa were surface sterilized in 70% ethanol or 5% Sodiume hypochlorite, then healthy seedlings were produced. The jemol, Hypocoty1 roo...
متن کاملThe Effect of Copper Sulphate on the Production of Flavonoids in Digitalis Lanata Cell Cultures
The paper investigated the effect of abiotic elicitor copper sulphate on the production of flavonoids in cell cultures of Digitalis lanata. Two cell lines were used (line 11 and line 13C-100) and two types of experiments were performed. In the first type of experiment the highest production of flavonoids was established, for both cell lines, for the strongest elicitor concentration (8μM). In th...
متن کامل